http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-005164-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_286d5c0dae7ad21e9e58e736d163f9eb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 |
| filingDate | 2000-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82e3575565df758d4bcd751ee372d659 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d3dc0a5d9fd7cf78857f7bca85a5224 |
| publicationDate | 2004-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-005164-B1 |
| titleOfInvention | METHOD AND SET OF REAGENTS TO DETERMINE THE ACTIVITY OF 5'-NUCLEOTIDASE |
| abstract | The invention relates to a method for determining the activity of 5'-nucleotidase, in which a biological sample is incubated according to the method and under conditions known per se, with nucleotide pentose monophosphate as a substrate, the released inorganic phosphate is converted into a colored complex by treating it with ammonium molybdate and a reducing agent, the color intensity is measured in a known manner and from the measured value of the 5'-nucleotidase activity or the amount of inorganic phosphate released per unit time, a value proportional to the 5'-nucleotidase activity is calculated using known calculations and/or using a calibration curve. According to the invention, 5'-AMP, 5'-CMP, 5'-UMP, 5'-GMP, 5'-IMP and 5'-TMP are used as nucleotide pentose monophosphates and measurements are performed on all six substrates. The invention also relates to a set of reagents for carrying out the above method. |
| priorityDate | 2000-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1449.