| abstract |
Fungal cells with genetic modifications for reduced O-glycosylation are new, Modifications are pref. in genes encoding enzymes of the o-glycosylation cycle, e.g. the Dol-P-Man:protein (Ser/Thr) mannosyl transferase DPM2, having the sequence given in the fif. The fungi pref. are flamentous fungi, e.g. Aspergillus, trichoderma, Mucor, Neurospora and Fusarium and yeasts, e.g. Kluyveromyces, Saccharomyces, Picha, hansenula and Candida. Also claimed is a DNA fragment encoding an enzyme for the transfer of mannosyl residues to the hydroxyl gp. of Seryl- or Threnoyl amino acids and its use as a probe for the isolation of homologous genes. |