http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-4224738-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_078e9c20d76969a8eacd242562ff1c8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a182c17c279e9c49dacb95612e50375b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6813 |
| filingDate | 1992-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef70213df4510b93a852be6c54e3c19b |
| publicationDate | 1994-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-4224738-A1 |
| titleOfInvention | Methods for the analysis of molecular organic structures |
| abstract | Analysis of organic structures, esp. nucleic acids, comprise (1) synthesis of non-radioactively labelled probes (A); (2) hybridisation to attach (A) to structures being examined and (3) detecting structural regions which have become attached to (A) using enzyme-conjugate complexes which generate chemiluminescence from a substrate. The new features are that (a) for detection a non-physiological solvent contg. a detergent is used and/or (b) for hybridisation the soln. used contains apart from Na2HPO4 and EDTA, a high concn. of detergent and less than 1% blocking reagent. USE/ADVANTAGE - The method is esp. used for Northern blotting analysis of nucleic acid but can also be applied to proteins. The method is sensitive, does not require radioactive materials and produces results quickly with a good signal-to-noise ratio. Use of the specified solns. reduces the background caused by non-specific binding. |
| priorityDate | 1992-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 179.