| abstract |
Method for DNA base sequencing comprises, (1) cloning a no. of DNA samples in different vectors (1-4), (2) synthesising the complementary strands of the samples (5-8) in admixture with primers (9-12) each primer being able to hybridise with only one particular vector and each labelled with different fluorophores having different emission wavelengths, (3) prepn. of 4 DNA-fragment gp. each with different end gps. from the resulting mixts. (i.e. 5-9, 6-10, 7-11, 8-12) and (4) electrophoretic migration of the fragment gps. and determination of fluorescence emission from the fragments in each gp. USE/ADVANTAGE - This method does not require complex sample pretreatments and, unlike the conventional multiplex DNA sequencing procedure, is not limited to samples which can be labelled with radiosotopes. Many samples can be processed simultaneously and no correction is required for differences in electrophoretic migration rates caused by different labelling dyes. |