http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-3816934-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_73d07e5d689c8b3391043d29fa83e911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b879dd2697afe455dbb78433f656b833 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82c2d1145ff9496c8574bfb4816a97bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c6ba594afc7a096ceb1ca4611f69b3e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 1988-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_990b2c7fb022ae60eff614208c814aae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f2592dadd5030aaf3c7f48e78c5b854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51249e6c0fe8eb39d0f80e0b720c3bc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c19cac10f42cbf93ee2898f72e54f0cf |
| publicationDate | 1989-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-3816934-A1 |
| titleOfInvention | NEW SEQUENCING METHOD FOR SINGLE OR DOUBLE-STROKEN NUCLEINE ACIDS AT FIXED PHASE |
| abstract | The invention includes a method and apparatus for the sequence analysis of single- and double-stranded nucleic acids on solid phase supports whereby nucleic acids for the purpose of sequencing are terminally elongated with modified nucleic acid units, which themselves serve as units for anchoring to a water insoluble and solid polymer support. In the preferred mode, modified nucleotides which are to be attached to a nucleic acid chain by chemical or enzymatically catalyzed reactions are in particular 5-bromo-2'-deoxyuridylate or in general other nucleotides halogenated or otherwise appropriately substituted at the base or sugar moieties, or streptavidin. Nucleic acids which can be immobilized in this way are either single-stranded DNA- or RNA-chains or such chains as parts of the double-stranded DNA or RNA. Water insoluble and solid supports are polymers such as cellulose, sepharose or sephadex as well as inorganic supports, in particular supports which have a backbone structure formed mainly by silicon and oxygen atoms. The water-insoluble solid supports have effector groups, in particular, they may contain immobilized antibodies specific to 5-bromo-2'-deoxyuridylate or other appropriate units capable of selective interaction with other halogenated nucleotides or nucleotides appropriately substituted at the base or sugar moieties. The binding of the nucleic acid strands which are to be sequenced to the polymer support is effected by interactions of the modified nucleotide units with the effector groups immobilized to the support, in particular by receptor ligand interactions. An apparatus is disclosed for carrying out the method. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8361717-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9308305-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0668867-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0668867-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2256217-A1 |
| priorityDate | 1988-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.