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filingDate 1978-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1978-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-2805029-A1
titleOfInvention PREVENTIVE AGENTS AGAINST VIRAL HAEMORRHAGIC SEPSIS AND TROUT, AND THEIR PRODUCTION AND USE
abstract Prodn. of a compsn. for preventing viral haemorrhagic septicaemia (VHS) of trout comprises first culturing the virus on fish cells, then inactivating it totally with beta-propiolactone (I). The virus used is pref. cultured on the EPC (epithelium papulosum cyprini) cell line to a titre of 104-109, esp. 5x107 to 5x108, plaque- forming units per ml. After deactivation with (I) the safety of the prepn. is pref. tested by 2 cultures on fish cell lines, and then opt. further treated with aq. HCHO to a final HCHO concn. of 0.05%. The vaccine protects trout again VHS more conveniently than the rigorous disinfection procedures normally used, and long-lasting protection is afforded.
priorityDate 1977-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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