| abstract |
Sepn. of enzymes from cell (residues) is carried out by solubilising the enzyme and partitioning the enzyme, together with insoluble cell materials and cell fluid, between the various phases of an aq. multiphase system contg. either (a) >=1 high-mol. -wt. cpd. (e.g. an opt. substd. polyol; polyether, polyester, polyvinylpyrrolidone or polys saccharide) and at >=1 inorganic salt, or (b) >=2 high-mol. wt. cpds. The phases are then sepd., and the enzyme is sepd. from the high-mol. -wt. cpd. and opt. isolated. The process can be used to separate, e.g. aminoacyl t-RNA synthetases from E. coli, maltose from brewer's yeast, or pullulanase from Klebsiella pneumoniae. It is not necessary to remove the cell fluid before the multiphase partition treatment. |