http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-2149666-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b89de41255af3e172bc770a76985c21e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| filingDate | 1971-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db31e45fbb7b797ba02bf54f5ab88435 |
| publicationDate | 1973-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-2149666-A1 |
| titleOfInvention | PROTEIN-LIKE MATERIAL FROM SCHISTOSOMA AND THIS DIAGNOSTIC PREPARATION |
| abstract | A process for isolating a globinolytic enzyme protein material from adult Schistosoma worms esp. of the genus mansoni which may be used as a diagnostic agent for Schistosoma infection, consists in lyophilising the washed worms, homogenising in a buffer, centrifuging, gel filtering through a membrane retaining mols. of M.W. 10,000, concentrating the retained supernatant, chromatographing on Sephadex G-200, eluting and measuring the absorption at 280 nm, and grouping the eluted fractions into 3 groups with peaks eluted at 0.5, 0.8 and 1.0 column vols. of eluate. The second of these groups contains the immunologically active material which is further purified by chromatography on a phenylalanineagarose column and eluting first with Na acetate buffer of pH 3.94 which removes inactive material then with 0.2M-acetic acid of pH 3.0 which elutes the active protein enzyme. This is unstable but may be stabilised with protein stabilisers such as glycerine. The protein molecule contains 246 plus-or-minus 15 amino acid units acid units of 18 different amino acids and has mol. wt. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2447194-A1 |
| priorityDate | 1971-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.