http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-202016009003-U1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74936e49a77ba0928def46130724cfb8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-027 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D71-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2016-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-202016009003-U1 |
| titleOfInvention | Composition comprising in vitro transcribed RNA obtainable by a method for the production and purification of RNA with at least one step with a tangential flow filtration |
| abstract | A composition comprising in vitro transcribed RNA obtainable by a method comprising the steps of: A) providing DNA which codes for the RNA; B) in vitro transcription of the DNA in order to obtain a solution which comprises transcribed RNA; andC) diafiltration and / or concentration and / or purification of the solution comprising transcribed RNA by one or more tangential flow filtration (TFF) steps; wherein in step C) the method comprises at least one diafiltration step using TFF; wherein the at least one diafiltration step using TFF in step C) comprises diafiltration with an aqueous salt solution; wherein the aqueous salt solution comprises about 0.1 M alkali metal halide to about 1 M alkali metal halide; wherein the method in step C) comprises at least one further cleaning method before or after the one or more TFF steps; wherein the at least one further purification process is a reverse phase chromatography process; wherein, in the reverse phase chromatography method, a stationary reverse phase and a mixture of an aqueous and an organic solvent is used as the mobile phase for eluting the RNA; and wherein the at least one or more TFF steps comprise using a TFF membrane cartridge. |
| priorityDate | 2015-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 319.