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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01002
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filingDate 1970-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 1971-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-2013700-A1
titleOfInvention Stable formate - dehydrogenase prodn
abstract Stable prepns. of formate-dehydrogenase (used for quantitative determination of formate ions) are obtained from cultures of Pseudomonas oxalaticus by preliminarily removing formate ions from the bacteria mass; adding nicotinamide-adenine dinucleotide during the processing of the bacteria; carrying out the decomposition of the cells (by ultrasonic vibrations) in the presence of a buffer maintaining the pH between 5.5 and 6.5; and, finally, destroying the accompanying enzymes (which oxidise dihyro-nicotinamide-adenine dinucleotide) by lowering the pH to 4.5-5.5 by the addition of an acid.
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type http://data.epo.org/linked-data/def/patent/Publication

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