| abstract |
The present invention relates to a method for sequence-specific recombination of DNA in eukaryotic cells, comprising introducing into a cell a first DNA sequence, inserting a copy of the first DNA sequence, and performing the sequence-specific recombination by the action of a resolvase or its derivative. The present invention relates to a method for testing topoisomerase inhibitors in a eukaryotic cell, comprising introducing into a cell a first DNA sequence according to SEQ ID NO: 1, introducing into a cell a copy of the first DNA sequence according to SEQ ID NO: 1 in direct orientation, the introduction of a wilt-type resolvase, and addition of the topoisomerase inhibitors to be tested. |