| abstract |
The present invention relates to a method for the electrochemical detection of sequence-specific nucleic acid-oligomer hybridization events. DNA / RNA / PNA oligomer single strands, which are bound at one end to a conductive surface and linked at the other free end to a catalytically redox-active unit, serve as a hybridization matrix (probe). By treatment with the oligonucleotide solution to be examined (target), part of the single-stranded oligonucleotides is hybridized, as a result of which the electrical communication between the conductive surface and the catalytically redox-active unit, which is originally not or only weakly present, is increased. This enables the detection of a hybridization event using electrochemical methods such as voltammetry, amperometry, potentiometry or conductivity measurement. |