| abstract |
The invention relates to heart cells with pathological features, their development from embryonic stem cell lines and their use, in particular as a viable model for heart diseases (eg arrhythmia, hypertrophy, ischemia). Fields of application of the invention are pharmacology and medicine (cardiophysiology). DOLLAR A The aim of the invention is to obtain cell lines with pathological features which can be used for specific applications in pharmacology (screening of active ingredients) and medicine (development of therapeutic strategies) by suitable choice of the differentiation conditions. DOLLAR A Differentiated cardiac cells from embryonic stem cell lines as an in vitro model of heart disease (arrhythmia, hypertrophy, ischemia) according to the invention by embryoid body differentiation of ES cells, EC cells or vertebrate EC cells (including humans) in the Mass culture, in hanging drop or by other methods, the method being characterized in that during the differentiation agents are added or culture conditions are chosen which change the normal differentiation program such that: an altered development program is activated so that heart cells are differentiated, which have pathological features. |