http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-19600362-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f84cba617b5e30314b195a382ba96bd9 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
filingDate | 1996-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb05cc7df08ba97a4f4bd998709b712b |
publicationDate | 1997-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-19600362-A1 |
titleOfInvention | Methods for the detection and quantification of nucleic acid molecules |
abstract | The invention relates to a process for detecting and quantifying nucleic acid (=NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: a) heteroduplexes are formed in which nucleotides which are unpaired or, according to Watson and Crick, incorrectly paired are chemically reactive with a carbon di-imide compound; b) the single or double-stranded ribonucleic acid (=RNA) molecules or single-stranded deoxyribonucleic acid (=DNA) molecules contained in population (I) are transferred into a population (II) of double-stranded DNA molecules; c) the population (II) of double-stranded DNA molecules are subjected to denaturation and subsequent renaturation, to produce a mixture of homo and heteroduplex DNA molecules. To make it possible to quantify the NA molecules detected, it is proposed that a purification stage take place between steps a) and b) and between steps b) and c). |
priorityDate | 1996-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.