| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-525
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate |
2020-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c7e5d59b7f2190aa7efeee6faec0de2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aed52aaa0f3b8bb606a6f6c07eece824
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_745763a79fbc9710f8e20b75ba58c21c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd401488e45b804b705ec988026ff4e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62e6c7c4e04c55e7476bbebf3fa726e1 |
| publicationDate |
2022-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
DE-112020002327-T5 |
| titleOfInvention |
CHEMICAL CAPTING FOR DIE CHANGE |
| abstract |
Provided herein is a method for chemically capping polynucleotides with a 5'-monophosphate. In some embodiments, the method may include: combining an activated 5'-nucleoside mono- or polyphosphate with a polynucleotide population comprising polynucleotides having a 5'-monophosphate to produce a reaction mixture; and incubating the reaction mixture to produce reaction products comprising a polynucleotide and a 5' nucleoside cap linked by a 5'-5' polyphosphate bond. The chemical capping method described here can be integrated into a variety of cDNA synthesis methods. |
| priorityDate |
2019-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |