http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10393473-T5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88be7c70739cbff9c6e626fa5080fcd0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34b7291bf580f3b188818418c0aaf558 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6875 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-115 |
| filingDate | 2003-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e200338d14d4513b62109bae3c09f6a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ceb12025fbe03c0670daacc109366a5 |
| publicationDate | 2005-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10393473-T5 |
| titleOfInvention | Trap tagging: a novel method for the identification and purification of RNA-protein complexes |
| abstract | A method of purifying an in vitro formed RNA-protein complex comprising: (a) providing an RNA fusion molecule comprising a target RNA sequence and at least two different RNA markers, wherein at least one RNA marker reversibly interacts with a ligand; (b) contacting the RNA fusion molecule with a cellular extract; (c) providing conditions that allow the formation of an RNA-protein complex on the target RNA sequence, and (d) subjecting the RNA-protein complex to at least two different affinity purification steps, wherein each step comprises binding an RNA label to an affinity resin capable of selectively binding an RNA label, and eluting the RNA label after removing non-affinity resin bound substances from the affinity resin. |
| priorityDate | 2002-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 170.