http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10392538-T5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418 |
| publicationDate | 2005-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10392538-T5 |
| titleOfInvention | Method for the analysis of methylated nucleic acids |
| abstract | A method for analyzing methylation in a CpG position or multiple CpG positions to be analyzed in a nucleic acid sample comprising: a. Reaction of unmethylated cytosine bases in the nucleic acid sample by treatment with an agent, in uracil or another base which differs from cytosine in terms of its base-pairing behavior; b. Amplification of one or more nucleic acids of the treated sample in an amplification reaction wherein at least two oligonucleotide primer pairs are provided for each CpG position to be analyzed, wherein a primer pair preferably hybridizes in the case where the CpG of the treated nucleic acid in the original sample before the reaction was methylated, and further wherein the other primer pair preferably hybridized in the case where the CpG of the treated nucleic acid in the original sample was unmethylated before the reaction; c. Detection of the amplicons formed in the polymerase reaction in a quantifiable manner; and d. Determination of the degree of methylation in at least one CpG position of the nucleic acid sample. |
| priorityDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.