http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10392538-B4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418 |
| publicationDate | 2010-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10392538-B4 |
| titleOfInvention | Method for the analysis of methylated nucleic acids |
| abstract | A method for analyzing methylation in a CpG position or multiple CpG positions to be analyzed in a nucleic acid sample comprising: a. Reaction of unmethylated cytosine bases in the nucleic acid sample by treatment with an agent in uracil or another base which differs from cytosine in terms of its base pairing behavior; b. Amplification of one or more nucleic acids of the treated sample simultaneously in an amplification reaction wherein at least two oligonucleotide primer pairs are provided for each CpG position to be analyzed, a primer pair preferably hybridizing in the case where the CpG of the treated nucleic acid is in the original Sample was methylated prior to reaction, and further wherein the other primer pair preferably hybridized in the case where the CpG of the treated nucleic acid in the original sample was unmethylated prior to the reaction and wherein the amplicons formed by each of the primer pairs are detected by a detectable fluorescent label differ; c. Real-time detection of the amplificates formed in the polymerase reaction in a quantifiable manner; |
| priorityDate | 2002-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.