http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10317669-B4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46570326040ec3afb9917a7e7799019f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01J3-021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6421 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6441 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-0064 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01J3-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01J3-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-0076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01J3-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01J3-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01J3-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a33fc054b0fdafb866100c83ff9bd87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e0d94796655322912da04cbc514c0e9 |
| publicationDate | 2017-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10317669-B4 |
| titleOfInvention | Method for separating detection channels of a microscopic system |
| abstract | A method for separating detection channels (131, 132, 133), wherein a sample (15) is provided with at least two fluorescent dyes, characterized by the following steps: a) determining an emission spectrum (115, 116, 117) of the at least two fluorescent dyes, wherein the at least two fluorescent dyes in the sample (15) are excited with different wavelengths, wherein the number of different wavelengths determines the number of samples in the sample (15) provided fluorescent dyes does not exceed; b) determining the separation points (118) of the emission spectrum (115, 116, 117) with respect to the wavelength in order to assign the corresponding part of the emission spectrum (115, 116, 117) to a particular channel (131, 132, 133) and to this channel (131, 132, 133); and c) adjusting the separation of the at least two channels (131, 132, 133) such that the parts of the total emission spectrum (115, 116, 117) determined on the basis of the separating points (118) each have different channels (131, 132, 133) for detection wherein the separation points (118) of the parts of the emission spectrum are defined by the intersections (119) of the individual emission spectra (115, 116, 117) of each fluorescent dye provided in the sample, or determined from the difference between that in a Excitation wavelength measured emission spectrum (115, 116, 117 of the biological sample and an emission spectrum (110, 111, 112) of one of at least two fluorescent dyes in the sample at the same excitation wavelength, the integral of the square of sums is formed and the separation points (118) by the minimum of the integral. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102020127385-B3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102017221187-B4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102017221187-A1 |
| priorityDate | 2003-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.