http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10237691-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6e768a8682465f7601b9677b190fe9c4 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2002-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c08ce0dc939f12f1ab5a8320bbaa2618 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93f5dc176a018af3f02c6a2a32c13740 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac3a0fb6da921c11e7f1e05401020a7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d17b270bdffcbbe5876eda09478fe3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83ad4154d0b2f9e6102fd081afc69961 |
publicationDate | 2004-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-10237691-A1 |
titleOfInvention | Method for the detection of single nucleotide polymorphisms (SNP) in genes of drug metabolism and test kit for carrying out the method |
abstract | The invention relates to a simplified method for the detection of SNP in cytochrome P450 (CYP) genes in humans and a test kit with the following components: 1. newly developed synthetic oligonucleotides, which are suitable for amplifying gene sections in genes of drug metabolism using isoform-specific (IS-PCR) DNA-polymerase chain reaction technology (PCR) from genomic leukocyte DNA, 2. Components which enable single-stranded biotinated IS-PCR products to be selectively, thermostably and single-stranded immobilized on streptavidin-coated microtiter plates (SA-MTP), 3. Test-optimized, allele-specific, fluorescein isothiocyanate (FITC) labeled synthetic oligonucleotides that ensure reliable genotyping of the immobilized amplification products (amplicons) by hybridization, subsequent stringent washing and subsequent detection using fluorometry or photometry, or alternatively 4. Streptavidin-coated glass slides (SA chip) on which biotinated, isoform-specific, synthetic oligonucleotides are immobilized, which are hybridized with partially single-stranded PCR products that are produced by discriminatory extension of an allele-specific, terminally FITC-labeled oligonucleotide using Taq DNA polymerase and by subsequent stringent washing and subsequent detection using fluorometry or photometry, reliable genotyping of the ... |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006010266-A1 |
priorityDate | 2002-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 307.