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filingDate 2002-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73c46e4b38e7946fc98e844ae1eae96d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b46d73d142f59ac886831b055633e710
publicationDate 2003-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-10208399-A1
titleOfInvention Procedure for the qualitative and quantitative determination of genetic material
abstract The invention relates to a method for the qualitative and quantitative determination of genetic material which contains a known target sequence which is characteristic of the material and which can be present alongside or in a mixture with other genetic material, in which DOLLAR A is a suspension containing the genetic material with the Contains target sequence and optionally other genetic material, mixed with (1) a molar excess of a first oligonucleotide probe whose nucleotide sequence is complementary to the target sequence, and optionally (2) mixed with a molar excess of a second oligonucleotide probe whose nucleotide sequence is complementary to a nucleotide sequence, which is contained in all components of the entire genetic material to which the quantification is to refer, DOLLAR A makes the entire genetic material, insofar as it is double-stranded, single-stranded, the first oligonucleotide probe with the target sequence and, if appropriate, the tw If the oligonucleotide probe hybridizes with the nucleotide sequence contained in all components of the genetic material, DOLLAR A in the suspension determines the concentration (s) of the non-hybridized first and possibly second oligonucleotide probe and from the concentration or the concentrations the proportion of the genetic material with the target sequence the total genetic material to which the quantification should refer.
priorityDate 2002-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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