http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102015115836-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15a58266c099fd93ecb8ca9d5787ad12 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2015-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97795edb7e056ce3b0dcc5f77974fa08 |
publicationDate | 2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-102015115836-A1 |
titleOfInvention | Confirmatory test for primary nucleic acid amplificates in a continuous reaction mixture and immediate evaluation by means of immunochromatographic methods |
abstract | The present invention relates to a method for confirming amplified nucleic acid target sequence (target sequence), preferably from human samples, during a amplification reaction in a collective and continuous reaction batch as a one-pot process wherein the confirmation of the target sequence amplicon is obtained via a hapten-pair-labeled template artificial amplificate. In this case, the artificial template sequence is amplified by means of the 5'-cleavage products of the at least one target sequence-specific FEN probe and optionally labeled. The 5 'cleavage product of the at least one target sequence-specific FEN probe is obtained only when the FEN probe hybridizes with its target sequence-specific 3' sequence to a complementary sequence portion of the at least one target sequence. The detection of the obtained plurality of template amplificates takes place distinctly and preferably by means of immunochromatographic methods. |
priorityDate | 2015-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 125.