| abstract |
A method for the label-free detection of an analyte and an associated analysis device is provided. The method comprises the steps of flowing a suspended particle into a microchannel of a microchip, guiding the suspended particle into a holding structure in the microchip and holding the suspended particle in the holding structure, optically exciting the held particle, and measuring a first emission spectrum of the held particle, determining a first Resonance mode of the particle in the first emission spectrum, supplying the analyte to the held particle by means of a flow, re-exciting the held particle and measuring a second emission spectrum of the held particle, determining a second resonance mode of the particle corresponding to the first resonance mode of the particle in the second emission spectrum, and Determining a positional shift, a change in a half-width and / or a splitting of the second resonance mode relative to the first resonance mode. |