http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102011007991-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44df766f7fca9d1c4f11e8e597b09ef1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 |
| filingDate | 2011-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fde701e582516fb85b1a6abaef33add http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc174abe7321417a3a25bda923b866e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53ca335e30e36438f66c6e29a1530e79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d82225fdba54e471d3e9eb0c778e2f8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ae268d4a9e69b9fa561c1bbff3a5e40 |
| publicationDate | 2013-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102011007991-A1 |
| titleOfInvention | Process for the fermentative production of D-phenylglycine |
| abstract | The present invention relates to a process for the fermentative preparation of D-phenylglycine (D-Phg) in enantiomerically pure form. For this purpose, genes pgIA, pgIB, pgIC, pgID and a gene coding for the stereoinverting D-aminotransferase gene are expressed in a recombinant cell. These genes are, in particular, the genes pgIA to pgID from S. pristinaespiralis, as well as the hpgAT gene from Pseudomonas putida. In this method, in the first step, the phenylpyruvate is converted by the pyruvate dehydrogenase-like complex PgIB / C to phenylacetyl-CoA. In the following, the dpg-dehydrogenase-like enzyme PgIA catalyzes the conversion of phenylacetyl-CoA to benzoylformyl-CoA. The Co-A residue is then replaced by the thioesterase PgID to form benzoylformate. In a final biosynthesis step, the stereoinverting D-aminotransferase transfers an amino group to benzoyl formate to form D-Phg. In particular, the recombinant cell may be a wild-type or mutant cell of Escherichia coli or a species of the order Actinomycetales. |
| priorityDate | 2011-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.