http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102010035003-B4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8334ae4110e6503bf99c14904012d49a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-648 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B21-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G02B27-56 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G02B21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2010-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e58b45a19c3cbed86bcb8b8f66d99cb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6effea2aef8f97683f603edb479fbed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fc86bff4fe0ff59ff05ce1e09f2b440 |
| publicationDate | 2015-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102010035003-B4 |
| titleOfInvention | Spatially and temporally high-resolution microscopy |
| abstract | Method for spatially and temporally high-resolution detection of the fluorescence of a fluorophore of a sample, comprising the following steps: a) the sample is placed on the surface of a sample carrier (14); b) adding to the sample a redox buffer comprising at least one reducing agent, and / or at least one oxidizing agent and / or at least one reducing-oxidizing agent to prevent photobleaching of the fluorophore; c) pulsed excitation light (10) with a pulse length less than 1 ns is coupled into the sample carrier (14) in such a way that it either irradiates the sample or leaves the sample carrier (14) only evanescently at the location of the sample; d) the wavelength of the excitation light (10) is selected such that the excitation light is capable of exciting the fluorescence of the fluorophore of the sample; e) fluorescent light emanating from the sample is guided with an optical structure (13, 24, 30) through a pinhole (32) onto a detector (34), wherein the optical assembly (13, 24, 30) by means of the pinhole (32) defines a detection volume (45); f) the detector (34) is selected so that it can detect individual photons of the fluorescent light with a temporal resolution of better than 1 ns; g) the fluorescent light is split and additionally recorded with a spatially resolving detector (22); h) by means of the spatially resolving detector (22) an interesting measurement region within the sample is identified; i) the spatially limited detection volume (45) of the time-resolved detection is positioned on the interesting measurement region in the sample; and j) the interesting measuring region is scanned with the spatially limited detection volume (45) of the time-resolved detection. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3648453-A1 |
| priorityDate | 2010-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.