| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2008-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66d8fbeb11c18f075c1893fd1f98eabb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8beee73526d52c731ac2e77686f433aa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c65f40622a9d1d424e2956969fdda5b |
| publicationDate |
2010-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
DE-102008063003-A1 |
| titleOfInvention |
Nucleic acid purification method |
| abstract |
The present invention relates to a method for purifying nucleic acids from a nucleic acid-containing sample, which comprises at least the following steps: a. Contacting the nucleic acid-containing sample with a protonatable nucleic acid-binding phase, the protonatable groups having a pKa of from 9 to 12; b. Binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) which is at least one pH unit below the pKa value of at least one of the protonatable groups; c. Elution of the nucleic acids at a pH which is above the formation pH, but at least one pH unit is below the pKs of at least one of the protonatable groups. In addition, corresponding kits as well as nucleic acid-binding phases which can be used for the purification of nucleic acids are disclosed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11104896-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017162518-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016198571-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013045432-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013045434-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9938520-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10184145-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11021736-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022263500-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021122846-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3412773-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4159857-A1 |
| priorityDate |
2008-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |