http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102008029999-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7a757a9e03318b537f71037eff5a2e5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2008-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2009-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102008029999-A1 |
| titleOfInvention | New PCR-based detection method to distinguish living from dead cells |
| abstract | Technical problem of the invention In connection with numerous z. B. medical or agricultural issues is of interest, not just the existence z. B. of microorganisms in samples to detect, but to be able to distinguish whether any microorganisms present are viable. By ordinary PCR methods this distinction is not made due to the high persistence of DNA. the solution of the problem The so-called splice PCR utilizes the much lower stability of RNA compared to genomic DNA to solve this problem. The use of at least one so-called splice primer ensures that an amplification product is formed only from RNA or from cDNA produced therefrom, but not from genomic DNA. The decisive factor here is that the sequence of the splice primer is complementary to the two-sided sequence context of an intron, so that a binding site for the primer is formed only in the context of the post-transcriptional modification of the RNA primary transcript. In the corresponding genomic DNA complementary to the splice primer binding site z. B. interrupted by an intron. field of use Evidence of the viability of microorganisms in z. B. food (quality control), medical or agricultural (efficacy efficiency of seed dressing) application contexts. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014015925-A1 |
| priorityDate | 2008-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.