http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102008013304-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e88eaf2501865c15f869b9915726ad9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2008-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77ba066f3a575e175d4c9fa6293e13ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8151229f947b8f334c69df9eb9387a63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d07242b8494db7c29640c3aeb80d82b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fa6dd063802fc35e5a56196b3049e15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a63b44201b28ae273b326e523e6679d9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6deac9bfa048d62bd495d27a8a7d16d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e58cac625f01ebded716653e5cfafbd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a1a411a538ec0c73f23f12130acb987 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_008618e3ec4358470b0a891e33532071 |
| publicationDate | 2009-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102008013304-A1 |
| titleOfInvention | Method and vector for heterologous gene expression |
| abstract | The invention relates to a method, an expression strain and a vector for the expression of heterologous genes. The method according to the invention comprises the following steps: - producing at least one expression vector; Cloning at least one heterologous target gene into the expression vector; - preparing a Rhodobacter capsulatus expression strain; Introducing the recombinant expression vector into the cells of the expression strain and - Expression of the heterologous target gene in the cells of the expression strain. The invention comprises Expressionsstammm Rhodobacter capsulatus cells of the bacterium, which are modified by integration of a T7 RNA polymerase gene is under the control of Rhodobacter-specific promoter P fru into the recA gene of Rhodobacter capsulatus chromosome. The vector of the invention is based on the plasmid pBBR22b, wherein the vector differs from pBBR22b by one or more of the following modifications: - deletion of the lacI gene; Insertion of the aphlI gene of the Tn5 transposon under the control of the Km promoter; Polylinker downstream of a T7 promoter and / or Polylinker downstream or upstream of the Km promoter of the aphl1 gene. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102010036997-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102010036997-A8 |
| priorityDate | 2008-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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