http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102007010142-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_10bd7baf82e51a4b37e0e24ee06d11ef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2007-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_087d01718471ec14a99eb7e58efb2eb2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f2927a7fafedf5c7481b08dee9f6786 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d23135ecefa0c89296d263fadd3d268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5399989312fbc3b38dfde4e325ea88f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a136afae58fb73d893bc3a9f978d6017 |
| publicationDate | 2008-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102007010142-A1 |
| titleOfInvention | Protein P4 (SLC10A4) as a marker for cholinergic neurons of the CNS |
| abstract | The present invention describes a method for detecting cholinergic neurons in tissue samples of the human or animal organism by detection of the protein SLC10A4. SLC10A4 occurs only in cholinergic neurons. In a preferred embodiment of the present invention, oligopeptides of the C-terminus of SLC10A4 are used to generate polyclonal antibodies. For this, amino acids 422-437 of the protein SLC10A4 are coupled to KLH at the carboxy-terminal glutamic acid residue. The KLH-coupled protein in this way is then injected into a vertebrate, which then forms polyclonal antibodies against this protein. Polyclonal antibodies to SLC10A4 obtained in this manner are used according to the invention to detect SLC10A4 in cholinergic neurons, for example by the sandwich method, in which, following incubation with the antibody of the invention, any second antibody is added by which the P4 Protein is optically visualized. The visualization of SLC10A4 can be done, for example, by immunofluorescence or peroxidase staining. |
| priorityDate | 2007-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.