abstract |
ThenThe present invention relates to a submersible process for the preparationnan enzyme comprising the steps of providing a minimal medium,nthe deionized water, mineral salts and an organic carbon sourcenhaving, in a vessel; of inoculationnthe minimal medium with a fungus; of incubating the minimal mediumnat a pH of 1-4, forna period of time sufficient for the fungus to be in the minimal mediumnis optically visible, as well as the recovery of the enzyme, wherein thenMinimal medium and the vessel is notnbe sterilized. Furthermore, the invention relates to an enzyme whichnby the method according to the inventionncan be produced. |