http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102006038113-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1373a00e11223d5b83c31a4ae3cb23db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135f30b72e489f20d4003baa11862aca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27e8364763696fd7a5c9b16cdb2b23ed |
| publicationDate | 2008-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102006038113-A1 |
| titleOfInvention | A method of synthesizing a cDNA in a sample in an enzymatic reaction and simultaneously providing a first enzyme having polyadenylation activity and a second enzyme having reverse transcriptase activity |
| abstract | ThenThe present invention relates to a process for synthesizing ancDNA in a sample in an enzymatic reaction, wherein the methodnincludes the stepsnsimultaneous provision of a first enzyme with polyadenylation activity, ansecond enzyme with reverse transcriptase activity, a buffer, at leastna ribonucleotide, at least one deoxyribonucleotide, onenAnchor oligonucleotide, adding a sample comprising a ribonucleic acid as wellnIncubation of the agents of the preceding steps at one ornseveral temperature steps, which are chosen so that the firstnand the second enzyme show activity.nThe invention further relates to a reaction mixture comprising anfirst enzyme with polyadenylation activity, a second enzyme with reservernTranscriptase activity, optionalna buffer, optionally at least one ribonucleotide, optionally at leastna deoxyribonucleotide and optional anchor oligonucleotide. moreovernThe invention relates to a kit comprising a corresponding reaction mixture. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016092062-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9096895-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010085966-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010085966-A2 |
| priorityDate | 2006-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 473.