http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102005060925-B4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eab56e4fe8837840887f41af83e328ee |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18a4a8ce1f447eaf9fa9e862b9d3303e |
| publicationDate | 2010-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102005060925-B4 |
| titleOfInvention | Method for the detection of DNA base sequence changes |
| abstract | A method of detecting DNA base sequence changes in a sample DNA comprising the steps a. Amplification of the sample DNA by polymerase chain reaction (PCR), b. Cleavage of the PCR product into fragments by a uracil-specific nuclease, c. mass spectrometric analysis of the fragments, d. Conclusion on the presence of a DNA base sequence change by comparison of the mass spectrometry determined fragment masses with fragment masses calculated for the presence of such DNA base sequence changes, characterized in that in step a. At least one of the primers used contains at least one position instead of one base, preferably in place of a thymine, a uracil, wherein the position of the uracil is selected so that after cleavage of the PCR product into fragments by a uracil-specific nuclease, the fragments, containing the DNA change to be detected, have a mass between 2000 and 6000 Da, The primers used for PCR bind to the regions of the sample DNA flanking the DNA base sequence change to be detected, - the PCR in the presence of dUTP, dATP, dGTP and dCTP ... |
| priorityDate | 2005-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 110.