http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102005060925-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eab56e4fe8837840887f41af83e328ee |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18a4a8ce1f447eaf9fa9e862b9d3303e |
| publicationDate | 2007-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102005060925-A1 |
| titleOfInvention | Method for the detection of DNA base sequence changes |
| abstract | The invention relates to a method for detecting DNA base sequence changes in a sample DNA comprising the steps a. Amplification of the sample DNA by polymerase chain reaction (PCR), wherein at least one of the primers used contains at least one position instead of one base, preferably instead of a thymine, a uracil, the primers used for PCR bind to the regions of the sample DNA flanking the DNA base sequence change to be detected, the PCR is carried out in the presence of dUTP, dATP, dGTP and dCTP, and a PCR product with a length of 33 to 60 base pairs is formed, b. Cleavage of the PCR product into fragments by a uracil-specific nuclease, c. mass spectrometric analysis of the fragments, d. Conclusion on the presence of a DNA base sequence change by comparison of the mass spectrometry determined fragment masses with fragment masses calculated for the presence of such DNA base sequence changes for use in all fields in which a rapid identification of small DNA sequence changes (mutations), such. Point mutations (base exchanges, single-base polymorphisms (SNPs), loss or insertion of a base) or small deletions or insertions (loss or insertion of fewer bases), e.g. B. Human and veterinary medicine (genetics, diagnostics of hereditary diseases, ... |
| priorityDate | 2005-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.