http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102005002978-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a854a1ac599b5f8d1cf6f0f2ee82d10 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 |
| filingDate | 2005-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ed54160af0a5f204839131a971b489d |
| publicationDate | 2006-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-102005002978-A1 |
| titleOfInvention | Recombinant expression of proteins in a disulfide-bonded, two-chain form |
| abstract | The present invention relates to a process for the preparation of polypeptides or proteins in two-chain form by means of recombinant expression in E. coli host cells, wherein (i) the polypeptide or protein exerts its biological activity as a two-chain polypeptide or protein, (ii) the C-terminal amino acid residue of the first chain is an Arg or Lys residue, (iii) the second chain of the protein / polypeptide has N-terminal 1 to 20 amino acid residues and a pentapeptide sequence designated VPX VPXGS, where X is any naturally occurring amino acid, wherein V Val, Leu, Ile, Ala, Phe , Pro or Gly, where P is Pro, Leu, Ile, Ala, Phe, Val or Gly, where G is Gly, Leu, Ile, Ala, Pro, Phe or Val and where S is Ser, Tyr, Trp or Thr ; and (iv) the method comprises the following steps: (a) modification of the polypeptide or protein, at the nucleic acid level, such that the polypeptide or protein in its modified form has in its loop region the sequence VPXGS, wherein X, V, P, G and S are as defined above are; (b) introducing the nucleic acid-level modified construct into E. coli cells; (c) culturing and then lysing the host cells and (d) Isolation of the two-chain polypeptide or protein. In a preferred embodiment, the polypeptide or protein is a botulinum neurotoxin, in particular the serotype A botulinum neurotoxin (BoNT (A)). |
| priorityDate | 2005-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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