http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102004001582-B4
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29bcad0ce19d5b8af84fc7a3847b11f8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2004-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2006-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_294f8841332ade394fbd730a6c0412c0 |
publicationDate | 2006-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-102004001582-B4 |
titleOfInvention | Method for producing DNA probes for DNA sections with repetitive sequences |
abstract | Process for the preparation of DNA probes with single-stranded DNA oligomers of a repetitive sequence motif, marked by Providing the single-stranded DNA oligomers of the repetitive sequence motif as a sense strand, Addition of complementary to the sense strand oligomers as Gegensinnstrang, Hybridize sense and antisense, Adding a first primer consisting of a first primer sequence and oligomers of the repetitive sequence motif, wherein the first primer sequence is not identical to the repetitive sequence motif, and adding a second primer consisting of a second primer sequence and the complementary to the sense strand oligomers of the repetitive sequence motif the second primer sequence is not identical to the repetitive sequence motif, Hybridize the primer, Adding nucleotides, a Klenow fragment and a ligase and allowing to react, Removing single-stranded DNA and DNA of less than 100 nucleotides in length, Amplification of the purified DNA by means of PCR and Label the PCR products with a reporter molecule. |
priorityDate | 2004-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.