http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10197267-T5

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D491-18
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D491-18
filingDate 2001-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39281510338d38bf941c194e4c7a6ca7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_234a209dbb2b7374f468691f14a7a04d
publicationDate 2005-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-10197267-T5
titleOfInvention A method for the extraction of tetrodotoxin
abstract Process for the extraction of tetrodotoxin from the tissue of an organism, comprising the following steps: a. Obtain a leached solution by aspirating the tissues with water and a weak organic acid, then filtering; b. Removing soluble proteins by heating the leached solution at a temperature below the boiling point; c. Obtaining an enriched tetrodotoxin solution by fixing the pH of the leached solution with a weak base water solution and then passing the solution through a cation ion exchange resin; d. Removing inorganic salts and alkaline amino acids by fixing the pH of the enriched tetrodotoxin solutions with an aqueous solution of a weak base, wherein the tetrodotoxin is absorbed by activated carbon and the tetrodotoxin is eluted from the activated carbon, and e. Crystallization of the tetrodotoxin by concentration of the eluate and determination of the pH of the eluate to a pH between 8 and 10.
priorityDate 2000-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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