http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10184524-T1

Outgoing Links

Predicate Object
assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c5e581037ef0fecbe64d8c79f9f7ccc
classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
filingDate 1996-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3a6797bcd9c6b551dc88ffdc8dd51d9
publicationDate 2011-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-10184524-T1
titleOfInvention DNA sequencing by parallel extension of oligonucleotides
abstract A method of identifying a sequence of nucleotides in a polynucleotide, the method comprising: (a) providing a first template polynucleotide comprising a binding region that includes a known sequence and a target region that includes an unknown sequence; (b) extending various sequence-initializing oligonucleotides by repeated cycles of duplex extension along a template, the method of extending a particular initializing oligonucleotide comprising: Forming a duplex between the first polynucleotide provided in step (a) and a second polynucleotide containing a sequence of the priming oligonucleotide, wherein the priming oligonucleotide hybridizes to a portion of the binding region of the first polynucleotide and Perform repeated duplex extension cycles that include: 1. providing a plurality of different oligonucleotide probes having different sequences; 2. Extend the second polynucleotide by ligating one of the various oligonucleotide probes with the second polynucleotide to produce a longer second polynucleotide, the probe being of the variety of different polynucleotides.
priorityDate 1995-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

Incoming Links

Predicate Subject
isDiscussedBy http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419584941
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID135398593

Total number of triples: 21.