http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10184524-T1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c5e581037ef0fecbe64d8c79f9f7ccc |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 1996-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3a6797bcd9c6b551dc88ffdc8dd51d9 |
publicationDate | 2011-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-10184524-T1 |
titleOfInvention | DNA sequencing by parallel extension of oligonucleotides |
abstract | A method of identifying a sequence of nucleotides in a polynucleotide, the method comprising: (a) providing a first template polynucleotide comprising a binding region that includes a known sequence and a target region that includes an unknown sequence; (b) extending various sequence-initializing oligonucleotides by repeated cycles of duplex extension along a template, the method of extending a particular initializing oligonucleotide comprising: Forming a duplex between the first polynucleotide provided in step (a) and a second polynucleotide containing a sequence of the priming oligonucleotide, wherein the priming oligonucleotide hybridizes to a portion of the binding region of the first polynucleotide and Perform repeated duplex extension cycles that include: 1. providing a plurality of different oligonucleotide probes having different sequences; 2. Extend the second polynucleotide by ligating one of the various oligonucleotide probes with the second polynucleotide to produce a longer second polynucleotide, the probe being of the variety of different polynucleotides. |
priorityDate | 1995-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419584941 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID135398593 |
Total number of triples: 21.