| abstract |
The invention relates to a method for expanding multipotent stem cells ex vivo. The invention further relates to a two-stage process for the expansion and differentiation of multipotent stem cells ex vivo, in which the stem cells at the first stage, i. H. can be gene transfected during the expansion phase. In the phase, the multipotent stem cells are differentiated into cells of the hematopoietic, endothelial or mesenchymal cell series. Stem and progenitor cells obtained in this way, as well as mature cells from the hematopoietic, endothelial and mesenchymal cell series, can be used, among other things, for the prophylaxis, diagnosis and therapy of human diseases and for tissue engineering. |