http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10158283-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate | 2001-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c73b92c6a3d8c6f422de1b247448909 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418 |
| publicationDate | 2003-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10158283-A1 |
| titleOfInvention | Method for the detection of cytosine methylation patterns with high sensitivity |
| abstract | A method is described for the detection of cytosine methylation in DNA samples, which consists of the following steps: First, a genomic DNA sample, which comprises DNA to be examined and background DNA, is chemically treated in such a way that all unmethylated cytosine bases in Uracil are converted, while the 5-methylcytosine bases remain unchanged. Then the chemically treated DNA sample is amplified using at least 2 primer oligonucleotides and a polymerase, the DNA to be examined being preferred over the background DNA as a template, and in the last step the amplified products are analyzed and from the presence of an amplified product and / or concluded from the analysis of further positions on the methylation status in the DNA to be examined. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10329240-A1 |
| priorityDate | 2001-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.