http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10155160-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50a20f10fb621bd7243040ce15cbd388 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-557 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-557 |
filingDate | 2001-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae225738234c88904b258a9bf4b6224b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3a6c6ad9f0cb4495358fdd123bad088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98e132fd68171a396dcee95d6b17c9a3 |
publicationDate | 2003-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | DE-10155160-A1 |
titleOfInvention | Bioanalytical measurement method for the determination of catalases and peroxidases, their conjugates, substrates, activators and inhibitors |
abstract | The invention relates to determination methods using enzymes from the group of catalases and peroxidases and using optical indicators from the group of europium-ligand complexes. The new method takes advantage of the surprising finding that the hydrogen peroxide (H¶2¶O¶2¶) bound as ligand by certain complexes of the Europium (III) ion is available for enzymatic reactions. The consumption of hydrogen peroxide caused by enzymes causes a change in the optical, in particular luminescence-optical properties of the europium complexes. With the help of the new reagents, catalases or peroxidases, but also their substrates, their inhibitors and activators, can be detected or determined in an improved manner. By using catalases or peroxidases as markers in immunological or genetic detection methods, antigens and nucleic acid oligomers can also be detected or determined. DOLLAR A The advantage of the indicators according to the invention lies in their great selectivity, Stokes shifts and long decay times. This offers the possibility of decelerating a disturbing background fluorescence first using a time-resolving measurement and only then determining the fluorescence of the EuTcWP indicator, which leads to extremely low detection limits in bioanalytics. |
priorityDate | 2001-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 271.