http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10154317-B4

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827
filingDate 2001-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2005-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf29d5598a285fae302042e1fb829c6c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418
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publicationDate 2005-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber DE-10154317-B4
titleOfInvention Method for the detection of cytosine methylations in immobilized DNA samples
abstract Method for analyzing cytosine methylation patterns in genomic DNA samples, comprising the following steps: a) the genomic DNA is isolated from cells or other accompanying materials and bound substantially irreversibly to a surface, b) the DNA bound to the surface is treated, preferably with a bisulfite, such that cytosine is converted into a base which is different from the base pairing behavior in the DNA duplex, while 5-methylcytosine remains unchanged, c) the reagents used in step b) are removed in a washing step, d) selected portions of the immobilized DNA are amplified in a polymerase reaction, e) the amplificates are examined with regard to their sequence.
priorityDate 2001-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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