http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10119203-B4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1231b3748b8fba87ad3bfd765885c588 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2001-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94e1ef29225381dff9bfa825128de5cd |
| publicationDate | 2004-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DE-10119203-B4 |
| titleOfInvention | Process for the production of double-stranded nucleic acids with single-stranded overhangs of any length and nucleotide composition |
| abstract | Process for the production of deoxyribonucleic acid (DNA) with one or two 5 'protruding ends, characterized in that DNA synthesis with one or two polymerase-resistant oligonucleotides is carried out by a DNA polymerase on a DNA template, with a polymerase-resistant oligonucleotide at least one Contains nucleotide analogue at any position that does not normally occur in DNA and the nucleotide analogue thereby fulfills the following requirements: a) If a free or a polymerase-resistant oligonucleotide already built into a DNA strand serves as a template for DNA resynthesis by a DNA polymerase, a 5'-overhang arises, which begins with the nucleotide analog and extends to the 5'-end of the polymerase-resistant Extends oligonucleotide. b) The nucleotide analog of the polymerase-resistant oligonucleotide is able to form a nucleotide-specific base pairing with deoxyribonucleic acid. c) The nucleotide analog of the polymerase-resistant oligonucleotide is able to enter into a nucleotide-specific base pairing with ribonucleic acid. d) The nucleotide analogue of the polymerase-resistant oligonucleotide does not impair the property of the polymerase-resistant oligonucleotide of being able to serve as the starting molecule for a new DNA synthesis. |
| priorityDate | 2001-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 384.