| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2000-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c73b92c6a3d8c6f422de1b247448909
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4903a12cd99a69496919bd211597d418
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cc6cfbf804d3ee9f3f0952afbe1704f |
| publicationDate |
2002-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
DE-10044543-A1 |
| titleOfInvention |
Method for determining the degree of methylation of certain cytosines in genomic DNA in the context of 5'-CpG-3 ' |
| abstract |
A method is described for the detection of the degree of methylation of a specific cytosine in the sequence context 5'-CpG-3 'of a genomic DNA sample. In the first step, the genomic DNA is chemically treated in such a way that the cytosine bases are converted into uracil, but not the 5-methylcytosine bases. Parts of the genomic DNA are then amplified, which contain said cytosine, the amplicons being given a detectable label and in the following steps the extent of hybridization of the amplicons to the two classes of oligonucleotides is determined by detecting the label of the amplicons and from the Ratio of the labels detected on the two classes of oligonucleotides as a result of the hybridization to the degree of methylation of the particular cytosine in the genomic DNA sample. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3382033-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-10255104-A1 |
| priorityDate |
2000-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |