| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f60f7884c3b8246b6939e7ca6e98d356 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-52 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-534
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8241 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-534
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 |
| filingDate |
2001-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a8d63044af74a39e45ef1d3ea34d6a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b0079becc29f9bee183532cd1e7444d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7012dcbd906497cc1a2289e7bb6bc61d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc6fea64b137f1e5817e627c8cca14ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ce5f12f428accb3f9a632cdc47fe780 |
| publicationDate |
2004-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
DE-01979431-T1 |
| titleOfInvention |
CONSTRUCTION OF WHOLE CELLS THROUGH MUTAGENESE OF A SIGNIFICANT PART OF AN INITIAL GENE, COMBINATION OF MUTATIONS AND, IF REQUIRED, REPEAT |
| abstract |
A method of identifying proteins by differently labeling peptides, the method comprising the following steps: (a) providing a sample comprising a polypeptide; (b) Providing a plurality of labeling reagents that differ in molecular weight, that can produce differently labeled peptides, that do not differ in their chromatographic retention properties, and that do not differ in their ionization and detection properties in mass spectrographic analysis distinguish, the molecular mass differences are recognizable by mass spectrography analysis; (c) fragmenting the polypeptide into peptide fragments by enzymatic digestion or by non-enzymatic fragmentation; (d) contacting the labeling reagents of step (b) with the peptide fragments of step (c) to label the peptides with the different labeling reagents; (e) separating the peptides by chromatography to produce an eluate; (f) feeding the eluate from step (e) into a mass spectrometer and quantifying the amount of each peptide and generating the sequence of each ... |
| priorityDate |
2000-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |