http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-301485-A7
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eab56e4fe8837840887f41af83e328ee |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 1989-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7509ef0df289e20447c9321eff6182da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ced97b7e82a455bb9dc0471447add04a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5c81ca9afc4fd4ca569c507634ee0c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1a3995ccbc9a6b09fedde9c7383ea9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2d78945e5e4f1bf9c3ff34739123383 |
| publicationDate | 1993-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-301485-A7 |
| titleOfInvention | HEF-CELL CULTURE FOR THE SELECTIVE RELEASE OF PROTEINS AND METHOD THEREFOR |
| abstract | The cell walls and cell membranes of the yeast cells are to be altered in a way that allows the passage of proteins up to a certain molecular weight after the 'pore-sieve effect', while larger cells are retained. The yeast cell culture consists of the strain HGY 51 (IMET 43912). The method is characterized in that yeast cells are treated with toluene and thiol compounds so that proteins whose molecular weight does not exceed 200 kDaltons are selectively released from the cells to the surrounding medium. The invention supports the recovery of leaked and enriched proteins without expensive cell digestions and separation processes or the enrichment or prepurification of the proteins remaining in the cell envelope for subsequent digestion. {Yeast; Cell disruption; Protein purification; Fermentation; toluene; thiol compounds; cell permeabilization} |
| priorityDate | 1989-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 707.