http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-299440-A5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62b563909ed77d94afc3c237e0fbd935 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 1989-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52d71b8ca43ae96ab4a8cf07efa19696 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2887ece3bee46f55d30214d63bb05295 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40b2584ba5e4f6479fe48f22b7e46c7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03d1ac28060edeeff7c4c9b28d1aedd1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47ceedb39535da7e0661963700c91198 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42a5e477fab85932b8e50961a6a7614a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a75d4cd83d09d2f8a9ee6786e42706e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49db08b58ecbfa2cfeae86ea95a50d35 |
| publicationDate | 1992-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-299440-A5 |
| titleOfInvention | METHOD FOR THE COMPLETE RESTRICTION OF DNA MOLECULES |
| abstract | The invention relates to a process for the complete restriction of DNA molecules. The invention relates to the field of genetic engineering, where a specific fragmentation of DNA molecules by means of restriction endonucleases belongs to the basic methods. The object of the invention is to completely and rapidly break down DNA molecules which were only incompletely cleavable using certain previously known restriction endonucleases using the previously known method into specific restriction fragments. The object was achieved by incubating such DNA molecules with the restriction endonuclease in the presence of a second DNA species (in particular oligonucleotide duplexes which contain the recognition site for the corresponding restriction endonuclease). The method for completing and accelerating the specific cleavage of phage lambda DNA by the restriction endonucleases Eco57I and Cfr9I, DNA of the phage vector mp18 by EcoRII and the plasmid pBR322 by NarI proved to be very advantageous. {Genetic engineering; restriction endonucleases; DNA; DNA cleavage; oligonucleotides; phage; vectors; plasmids; Cfr9I} |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0601834-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5422253-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5871911-A |
| priorityDate | 1989-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID23677676 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID450648135 |
Total number of triples: 23.