http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-296805-A7
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84f626c67a21089e624199aa26a0d1d4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
| filingDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a23f785bb587afd0947e8d5feb3efff4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43500ac3e23cad655644fa1ccd8c55ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b04752a905f256321a7b36b6e5a310b |
| publicationDate | 1991-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-296805-A7 |
| titleOfInvention | METHOD FOR INCREASING THE THERMOSTABILITY OF URATOXIDASE |
| abstract | The invention relates to a method for increasing the thermal stability of urate oxidase by gentle modification. By this method activity losses of particular fungal urate oxidase during transport, storage and application are significantly reduced. According to the invention, the gentle modification of the urate oxidase with dicarboxylic acid diimidoesters in the presence of purine or s-triazine derivatives in borate buffer is carried out at pH 9-10. The reaction is carried out after max. Stopped for 15 min by addition of a lower aliphatic primary amine. As purine or triazine derivatives are preferably xanthine or the potassium salt of allantoxan (0.5 to 5 mmol / l) application. The diimidoesters preferably have a chain length of 5 to 8 carbon atoms. They are used in a concentration of 1 to 10 mmol / l. Adipic diimidoesters are preferably used in a concentration of 6 mmol / l. {Thermostabilitaetserhoehung; fungal urate oxidase; gentle modification; Reduction of activity losses; Dicarbonsaeurediimidoester; purine derivatives; xanthine; s-triazine derivatives; Allantoxansaeure; Adipinsaeurediimidoester} |
| priorityDate | 1988-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.