http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-296701-A5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_19b5fafdbef7dff40427e093006e4405 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-73 |
| filingDate | 1990-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46fd245ef5c80e04b97aec4d7ddb3ce5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0383193f389c9e1f07b9ed5a4551e19 |
| publicationDate | 1991-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-296701-A5 |
| titleOfInvention | REGULATORY EUKARYOTIC HOST VECTOR SYSTEM BASED ON THE CI [EXPTS] REPRESSOR SYSTEM OF E. COLI-PHAGEN LAMBDA, METHOD FOR THE PRODUCTION AND USE THEREOF |
| abstract | An operator of the E. coli phage lambda cIts repressor is modified so that there is a consensus sequence between the two binding sites of the repressor molecules for a TATA box (especially the yeast), but the binding affinity of the repressor to the so modified operator is not may change. This modified operator is incorporated in place of or downstream of the natural TATA box or functionally similar regions of any eukaryotic promoter and transferred into a eukaryotic host previously altered to produce cIts repressor molecules by means of a vector. The original transcriptional strength of the eukaryotic promoter is not impaired. Depending on the synthesis of cIts repressor molecules, transcription of the eukaryotic promoter is repressed with the modified operator. The repression of transcription by the cIts repressor can be abrogated by a temperature increase to 42C and / or synthesis stop of the cIts repressor. {CIts repressor; Operator; eukaryotic promoter; Temperature regulation; S. cerevisiae; Glyceraldehyde 3-phosphate dehydrogenase promoter; regulatable expression vector; TATA box} |
| priorityDate | 1990-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 264.