| abstract |
A method of generating a large number of analogs of a selected peptide will be described. The method comprises the following steps: synthesizing two different mixtures of identically long oligonucleotides having different bases at some specific positions and some identical bases at the 3-end, mixing the two mixtures to form a mixture of partially double-stranded, equally long DNA sequences, which are then enzymatically converted into completely double-stranded DNA sequences, cutting the latter mixture with two different enzymes to produce DNA sequences that can be ligated to a similarly cut vector, inserting the DNA sequences into the vector by ligation to generate a mixture of vectors, which are then transformed into a host in a manner known per se, proliferating the host to form colonies which are analyzed sequentially to detect the DNA sequences encoding a single protein encode, under expression conditions separate growth of the large number of hosts to produce a large number of analogs of the chosen peptide. In addition, new analogs of VIP and a plasmid containing a gene encoding one of the novel analogs of VIP are disclosed. manufacture; Peptide; oligonucleotides; bases; 3 'end; DNA sequences; enzymes; Vector} |