http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DD-293652-A5
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8c96e1b5833e0a96da8d0d926d9f3eb |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-541 |
| filingDate | 1989-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f691c128e5911855546590a409f6dbbf |
| publicationDate | 1991-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | DD-293652-A5 |
| titleOfInvention | IMMUNOLOGICAL PROOF PROCESS FOR PHYTOHORMONE |
| abstract | The invention relates to an immunological method which allows the detection of phytohormones, preferably from the group of cytokinins, cost-effectively and with little time expenditure by means of indirectly competitive ELISA. Native serum is used according to the invention, with suitable measures, such as the direct coating of the microtest plate surfaces with a g-immunoglobulin hapten complex or the precoating with an antibody directed against the carrier protein of this complex, largely minimizing the influence of surface inhomogeneities in the microtest plates used. Hapten in the sample and the native serum antisera directed against it, digested in PBS containing calf serum, HSA and Tween 20, are incubated together at 4 ° C for 2 hours. The specific displacement of the antibodies from the hapten standard bound to the test plate surface is detected by means of a second enzyme-labeled antibody. Fields of application are plant physiology, microbiology and medicine. {Immunological detection method; phytohormones; cytokinins; indirectly competitive ELISA; G hapten-immunoglobulin complex; native serum; Abblockschritt; Antitraegerproteinantikoerper} |
| priorityDate | 1989-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.